[Product Usage]
This kit is used to determine the content of tetracycline in animal tissue, shrimp, honey and other related samples.
[Packaging Specifications]
48T/box, 96T/box
[Principle of experiment]
This kit adopts the indirect competitive ELISA method. The tetracycline antigen is pre-coated on the microwell strip of the ELISA plate, and the tetracycline remaining in the sample competes with the antigen pre-coated on the microwell strip to compete with the tetracycline antibody. The color of the substrate is developed, and the absorbance value of the sample is negatively correlated with its tetracycline. The content of tetracycline in the sample can be obtained by comparing it with the standard curve and multiplying its corresponding dilution ratio.
[Main components]
Table 1 Main components of tetracycline quantitative detection kit (enzyme-linked immunosorbent assay)
|
Components |
Specification |
Storage condition |
Type |
|
Antigen-coated ELISA plate |
96 holes |
+2 - +8℃ |
Removable and ready to use |
|
Sample Diluent (20×concentrate) |
15ml |
+2 - +8℃ |
High concentrated |
|
Washing solution (20×concentrated) |
30ml |
+2 - +8℃ |
High concentrated |
|
Tetracycline antibody solution |
7ml |
+2 - +8℃ |
Ready to use |
|
Tetracycline standard solution |
0.8ml/tube×5 |
+2 - +8℃ |
Ready to use |
|
High concentration tetracycline standard solution |
1ml |
+2 - +8℃ |
High concentrated |
|
Horseradish enzyme-labeled antibody working solution |
7ml |
+2 - +8℃ |
Working concentration, ready to use |
|
TMB substrate solution |
12ml |
+2 - +8℃ |
Single component, ready to use |
|
Stop solution |
12ml |
+2 - +8℃ |
Ready to use |
|
Sealing film |
3 |
|
|
|
Package Insert |
1 |
|
|
[Storage conditions and validity period]
The kit is stored at 2-8℃and is valid for 12 months.
