[Product Usage]
This kit is used to determine the content of sulfamethoxazole in animal tissues, milk, honey and other related samples.
[Packaging Specifications]
48T/box, 96T/box
[Principle of experiment]
This kit adopts the indirect competitive ELISA method, pre-coated sulfa-methoxine antigen on the microwell strip of the microtiter plate, the residual sulfa-paramethoxine in the sample and the pre-coated antigen on the microwell strip compete with sulfa-paramethoxine Pyrimidine antibody, after adding the enzyme-labeled secondary antibody, develops color by TMB substrate, the absorbance value of the sample is negatively correlated with its sulfanilamide to methoxine, and it is compared with the standard curve and then multiplied by its corresponding dilution factor, and the sulfanilamide in the sample can be obtained. The content of p-methoxypyrimidine.
[Main components]
Table 1. Main components of sulfa-methoxine quantitative detection kit (enzyme-linked immunosorbent assay)
|
Components |
Specification |
Storage condition |
Type |
|
Antigen-coated ELISA plate |
96 holes |
+2 - +8℃ |
Removable and ready to use |
|
Sample Diluent (20×concentrate) |
20ml |
+2 - +8℃ |
High concentrated |
|
Washing solution (20×concentrated) |
30ml |
+2 - +8℃ |
High concentrated |
|
Sulfonamide paramethoxine antibody solution |
7ml |
+2 - +8℃ |
Ready to use |
|
Sulfonamide-paramethoxine standard solution |
0.8ml/tube×6 |
+2 - +8℃ |
Ready to use |
|
High concentration sulfamethoxine standard solution |
1ml |
+2 - +8℃ |
High concentrated |
|
Horseradish enzyme-labeled antibody working solution |
7ml |
+2 - +8℃ |
Working concentration, ready to use |
|
TMB substrate solution |
12ml |
+2 - +8℃ |
Single component, ready to use |
|
Stop solution |
12ml |
+2 - +8℃ |
Ready to use |
|
Sealing film |
3 |
|
|
|
Package Insert |
1 |
|
|
[Storage conditions and validity period]
The kit is stored at 2-8°C and is valid for 12 months.
