[Product Usage]
This kit is used to determine the content of oxytetracycline in animal tissues, honey, shrimp and other related samples.
[Packaging Specifications]
48T/box, 96T/box
[Principle of experiment]
This kit adopts the indirect competitive ELISA method. The oxytetracycline antigen is pre-coated on the microwell strip of the ELISA plate, and the residual oxytetracycline and the antigen pre-coated on the microwell strip compete with the oxytetracycline antibody. After the secondary antibody was developed with TMB substrate, the absorbance value of the sample was negatively correlated with its oxytetracycline. The content of oxytetracycline in the sample could be obtained by comparing it with the standard curve and multiplying its corresponding dilution ratio.
[Main components]
Table 1. Main components of oxytetracycline quantitative detection kit (enzyme-linked immunosorbent assay)
|
Components |
Specification |
Storage condition |
Type |
|
Antigen-coated ELISA plate |
96 holes |
+2 - +8℃ |
Removable and ready to use |
|
Sample Diluent (20×concentrate) |
15ml |
+2 - +8℃ |
High concentrated |
|
Washing solution (20×concentrated) |
30ml |
+2 - +8℃ |
High concentrated |
|
Oxytetracycline antibody solution |
7ml |
+2 - +8℃ |
Ready to use |
|
Oxytetracycline standard solution |
0.8ml/tube×5 |
+2 - +8℃ |
Ready to use |
|
High concentration oxytetracycline standard solution |
1ml |
+2 - +8℃ |
High concentrated |
|
Horseradish enzyme-labeled antibody working solution |
7ml |
+2 - +8℃ |
Working concentration, ready to use |
|
TMB substrate solution |
12ml |
+2 - +8℃ |
Single component, ready to use |
|
Stop solution |
12ml |
+2 - +8℃ |
Ready to use |
|
Sealing film |
3 |
|
|
|
Package Insert |
1 |
|
|
[Storage conditions and validity period]
The kit is stored at 2-8℃and is valid for 12 months.
