General rules for ELISA experiments

1. To ensure the accuracy of the pipette gun, the error shall not exceed 2%. It can be determined by water and electronic balance. But it's best to have a professional to correct it.

2. One 20ul, 50ul, 100ul, 1000ul and one platoon gun shall be equipped. After absorbing different liquids, replace the gun head. Even when absorbing standards.

3. Take the kit out of the refrigerator one hour before the experiment and restore all reagents to room temperature to make the results more stable.

4. During the experiment, the substrate should be kept away from light.

5. When using the gun to absorb liquid, the speed should not be too fast, so as to avoid generating bubbles and making the absorption amount inaccurate.

6. When sucking liquid, use a gun with a range close to the required amount to reduce the error.

7. When the liquid is added into the enzyme labeling hole, avoid the contact between the gun head and the liquid in the hole, so that the droplets on the gun head can contact the hole wall, and the droplets will flow down naturally.

8. After all the liquid is added, shake the enzyme label plate in parallel and gently on the table for 30 seconds to mix the liquid. You can also use the shaking function of the enzyme labeling instrument.

9. During warm bath, the enzyme label plate shall be sealed with self-adhesive or adhesive tape to prevent evaporation of water.

10. When washing the plate, each time the washing solution is added, it should stand for 1 minute to make the cleaning more thorough. When there is no washing machine, after pouring the liquid, pat the enzyme label plate on the newspaper or wool paper.

11. When the lotion is not enough, use distilled water to prepare pH7.4, 0.02M phosphoric acid buffer, and add 0.1% Tween 20 as the lotion. It can be stored for a long time after adding 1 / 1000 sodium azide.

12. The substrate is light sensitive and should be prepared before use.

13. Before testing, turn on the enzyme labeling instrument to make it stable for more than 10 minutes.

14. The substrate has certain toxicity, and the termination solution is corrosive to the skin, so contact should be avoided as far as possible.

15. The samples to be tested shall be clarified, otherwise the results will be affected.

16. The warm bath time shall comply with the provisions of the kit.

17. Double hole experiment should be done as much as possible to ensure the accuracy of data.

18. Samples with doubts about the results shall be confirmed by other methods.