[Product Usage]
This kit is used to determine the content of gentamicin in cereals, flour and other related samples.
[Packaging Specifications]
48T/box, 96T/box
[Principle of experiment]
This kit adopts the indirect competitive ELISA method. The gentamicin antigen is pre-coated on the microwell strip of the ELISA plate. After adding the enzyme-labeled secondary antibody, the TMB substrate developed the color, and the absorbance value of the sample was negatively correlated with its gentamicin. Compared with the standard curve and then multiplied by its corresponding dilution, the concentration of gentamicin in the sample can be obtained. content.
[Main components]
Table 1. Main components of gentamicin quantitative detection kit (enzyme-linked immunosorbent assay)
|
Components |
Specification |
Storage condition |
Type |
|
Antigen-coated ELISA plate |
96 holes |
+2 - +8℃ |
Removable and ready to use |
|
Sample Diluent (20× Concentrate) |
15ml |
+2 - +8℃ |
High concentrated |
|
Washing solution (20×concentrated) |
30ml |
+2 - +8℃ |
High concentrated |
|
Gentamicin antibody solution |
7ml |
+2 - +8℃ |
Ready to use |
|
Gentamicin standard solution |
0.8ml/tube×6 |
+2 - +8℃ |
Ready to use |
|
High concentration gentamicin standard solution |
1ml |
+2 - +8℃ |
High concentrated |
|
Horseradish enzyme-labeled antibody working solution |
7ml |
+2 - +8℃ |
Working concentration, ready to use |
|
TMB substrate solution |
12ml |
+2 - +8℃ |
Single component, ready to use |
|
Stop solution |
12ml |
+2 - +8℃ |
Ready to use |
|
Sealing film |
3 |
|
|
|
Package Insert |
1 |
|
|
[Storage conditions and validity period]
The kit is stored at 2-8°C and is valid for 12 months.
