[Product Usage]
This kit is used to determine furazolidone (AOZ) content in animal tissues, milk and other related samples.
[Packaging Specifications]
48T/box, 96T/box
[Principle of experiment]
This kit adopts the indirect competitive ELISA method. Furazolidone antigen is pre-coated on the microwell strip of the enzyme labeling plate, and the furazolidone remaining in the sample competes with the antigen pre-coated on the microwell strip to compete with the furazolidone antibody. After adding the enzyme-labeled secondary antibody, the TMB The color of the substrate is developed, and the absorbance value of the sample is negatively correlated with its furazolidone. The content of furazolidone in the sample can be obtained by comparing it with the standard curve and multiplying its corresponding dilution ratio.
[Main components]
Table 1. Main components of Furazolidone (AOZ) Quantitative Detection Kit (ELISA)
|
Components |
Specification |
Storage condition |
Type |
|
Antigen-coated ELISA plate |
96 holes |
+2 - +8℃ |
Removable and ready to use |
|
Sample Diluent (20×concentrate) |
15ml |
+2 - +8℃ |
High concentrated |
|
Washing solution (20×concentrated) |
30ml |
+2 - +8℃ |
High concentrated |
|
Furazolidone (AOZ) Antibody Liquid |
5ml |
+2 - +8℃ |
Ready to use |
|
Furazolidone (AOZ) Standard Solution |
0.8ml/tube×6 |
+2 - +8℃ |
Ready to use |
|
High concentration furazolidone (AOZ) standard solution |
1ml |
+2 - +8℃ |
High concentrated |
|
Horseradish enzyme-labeled antibody working solution |
7ml |
+2 - +8℃ |
Working concentration, ready to use |
|
TMB substrate solution |
12ml |
+2 - +8℃ |
Single component, ready to use |
|
Stop solution |
12ml |
+2 - +8℃ |
Ready to use |
|
Derivatization reagent |
10ml |
+2 - +8℃ |
Ready to use |
|
Sealing film |
3 |
|
|
|
Package Insert |
1 |
|
|
[Storage conditions and validity period]
The kit is stored at 2-8°C and is valid for 12 months.
