[Product Usage]
This kit is used to determine the content of nitrofurazone (SEM) in animal tissues, milk and other related samples.
[Packaging Specifications]
48T/box, 96T/box
[Principle of experiment]
This kit adopts the indirect competitive ELISA method, pre-coating the nitrofuracil antigen on the microwell strip of the microtiter plate, the residual nitrofuracil in the sample and the antigen pre-coated on the microwell strip compete with the nitrofuracil antibody, after adding the enzyme-labeled secondary antibody, the TMB The color of the substrate is developed, and the absorbance value of the sample is negatively correlated with its nitrofurazone. Compared with the standard curve and then multiplied by its corresponding dilution factor, the content of nitrofurazone in the sample can be obtained.
[Main components]
Table 1. Main components of nitrofurazone (SEM) quantitative detection kit (enzyme-linked immunosorbent assay)
|
Components |
Specification |
Storage condition |
Type |
|
Antigen-coated ELISA plate |
96 holes |
+2 - +8℃ |
Removable and ready to use |
|
Sample Diluent (20×concentrate) |
15ml |
+2 - +8℃ |
High concentrated |
|
Washing solution (20×concentrated) |
30ml |
+2 - +8℃ |
High concentrated |
|
Furacillin (SEM) antibody solution |
5ml |
+2 - +8℃ |
Ready to use |
|
Furacillin (SEM) standard solution |
0.8ml/tube×6 |
+2 - +8℃ |
Ready to use |
|
High concentration nitrofurazone (SEM) standard solution |
1ml |
+2 - +8℃ |
High concentrated |
|
Horseradish enzyme-labeled antibody working solution |
7ml |
+2 - +8℃ |
Working concentration, ready to use |
|
TMB substrate solution |
12ml |
+2 - +8℃ |
Single component, ready to use |
|
Stop solution |
12ml |
+2 - +8℃ |
Ready to use |
|
Derivatization reagent |
10ml |
+2 - +8℃ |
Ready to use |
|
Sealing film |
3 |
|
|
|
Package Insert |
1 |
|
|
[Storage conditions and validity period]
The kit is stored at 2-8°C and is valid for 12 months.
