[Product Usage]
This kit is used to determine the content of enrofloxacin in animal tissue, milk, honey and other related samples.
[Packaging Specifications]
48T/box, 96T/box
[Principle of experiment]
This kit adopts the indirect competitive ELISA method. The enrofloxacin antigen is pre-coated on the microwell strip of the ELISA plate. After adding the enzyme-labeled secondary antibody, the TMB substrate was used for color development. The absorbance value of the sample was negatively correlated with its enrofloxacin. Compared with the standard curve and then multiplied by its corresponding dilution factor, the enrofloxacin in the sample can be obtained. content.
[Main components]
Table 1. Main components of Enrofloxacin Quantitative Detection Kit (ELISA)
|
Components |
Specification |
Storage condition |
Type |
|
Antigen-coated ELISA plate |
96 holes |
+2 - +8℃ |
Removable and ready to use |
|
Sample Diluent (20×concentrate) |
15ml |
+2 - +8℃ |
High concentrated |
|
Washing solution (20×concentrated) |
30ml |
+2 - +8℃ |
High concentrated |
|
Enrofloxacin Antibody Liquid |
7ml |
+2 - +8℃ |
Ready to use |
|
Enrofloxacin standard solution |
0.8ml/tube×6 |
+2 - +8℃ |
Ready to use |
|
High concentration enrofloxacin standard solution |
1ml |
+2 - +8℃ |
High concentrated |
|
Horseradish enzyme-labeled antibody working solution |
7ml |
+2 - +8℃ |
Working concentration, ready to use |
|
TMB substrate solution |
12ml |
+2 - +8℃ |
Single component, ready to use |
|
Stop solution |
12ml |
+2 - +8℃ |
Ready to use |
|
Sealing film |
3 |
|
|
|
Package Insert |
1 |
|
|
[Storage conditions and validity period]
The kit is stored at 2-8°C and is valid for 12 months.
