[Product Usage]
This kit is used to determine the content of doxycycline in animal tissue, shrimp, honey and other related samples.
[Packaging Specifications]
48T/box, 96T/box
[Principle of experiment]
This kit adopts the indirect competitive ELISA method. The doxycycline antigen is pre-coated on the microwell strip of the ELISA plate. The residual doxycycline in the sample and the antigen pre-coated on the microwell strip compete with the doxycycline antibody, and the enzyme label is added. After secondary antibody, color development by TMB substrate, the absorbance value of the sample is negatively correlated with its doxycycline, compared with the standard curve and multiplied by its corresponding dilution, the content of doxycycline in the sample can be obtained.
[Main components]
Table 1. Main components of Doxycycline Quantitative Detection Kit (ELISA)
|
Components |
Specification |
Storage condition |
Type |
|
Antigen-coated ELISA plate |
96 holes |
+2 - +8℃ |
Removable and ready to use |
|
Sample Diluent (20×concentrate) |
15ml |
+2 - +8℃ |
High concentrated |
|
Washing solution (20×concentrated) |
30ml |
+2 - +8℃ |
High concentrated |
|
Doxycycline antibody solution |
7ml |
+2 - +8℃ |
Ready to use |
|
Doxycycline standard solution |
0.8ml/tube×5 |
+2 - +8℃ |
Ready to use |
|
High concentration doxycycline standard solution |
1ml |
+2 - +8℃ |
High concentrated |
|
Horseradish enzyme-labeled antibody working solution |
7ml |
+2 - +8℃ |
Working concentration, ready to use |
|
TMB substrate solution |
12ml |
+2 - +8℃ |
Single component, ready to use |
|
Stop solution |
12ml |
+2 - +8℃ |
Ready to use |
|
Sealing film |
3 |
|
|
|
Package Insert |
1 |
|
|
[Storage conditions and validity period]
The kit is stored at 2-8°C and is valid for 12 months.
