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Shandong BioTruthSearch Co., Ltd.
Contact:Ms. Yan
TEL:13021789397
E_mail:info@biotruthsearch.com
Website:en.biotruthsearch.com
Add:Innovation Building, No. 22 Jinyu Road, Jining High-tech Zone, Shandong Province
Human C-reactive protein Immunoassay Kit
Release time:2022-05-25 09:47:36

[Packing Specifications] 48T/box, 96T/box

[Intended use] Human C-reactive Protein Immunoassay Kit is used to detect the content of C-reactive protein in human blood/plasma in vitro.

[Principle of experiment] This kit uses the double antibody sandwich method to determine the level of human C-reactive protein (CRP) in the specimen. Coat the microplate with purified human C-reactive protein (CRP) antibody to make a solid-phase antibody. Add human C-reactive protein (CRP) to the microwells coated with monoclonal antibody in turn, and then combine with HRP-labeled human C. Reactive protein (CRP) antibody binds to form antibody-antigen-enzyme-labeled antibody complex, which is washed thoroughly and then added substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The shade of color is positively correlated with the human C-reactive protein (CRP) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the concentration of human procalcitonin (PCT) in the sample was calculated from the standard curve.

[Main components] Table 1. Human procalcitonin immunoassay kit (PCT enzyme-linked immunosorbent assay)

Components

Specification

Storage condition

Type

Antibody-coated ELISA plate

96 holes

+2 - +8°C

Removable, ready-to-use

Sample Diluent

15ml

+2 - +8°C

Ready to use

Washing solution (30×concentrated)

30ml

+2 - +8°C

High-concentrated

Human C-reactive protein (CRP)

0.1ml

+2 - +8°C

High-concentrated

Horseradish enzyme-labeled antibody working solution

15ml

+2 - +8°C

Working concentration, ready to use

TMB substrate solution

15ml

+2 - +8°C

Single component, ready to use

Stop solution

15ml

+2 - +8°C

Ready to use

Sealing film

3

Package Insert

1

[Storage conditions and validity period] The kit is stored at 2-8°C, and the validity period is 12 months.

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