[Product Usage]
This kit is used to determine the content of chlortetracycline in animal tissue, shrimp, honey and other related samples.
[Packaging Specifications]
48T/box, 96T/box
[Principle of experiment]
This kit adopts the indirect competitive ELISA method, pre-coating the chlortetracycline antigen on the microwell strip of the ELISA plate, the residual chlortetracycline in the sample and the antigen pre-coated on the microwell strip compete with the chlortetracycline antibody, and adding the enzyme label After secondary antibody, color development by TMB substrate, the absorbance value of the sample is negatively correlated with its chlortetracycline, compared with the standard curve and multiplied by its corresponding dilution, the content of chlortetracycline in the sample can be obtained.
[Main components]
Table 1. Main components of chlortetracycline quantitative detection kit (enzyme-linked immunosorbent assay)
|
Components |
Specification |
Storage condition |
Type |
|
Antigen-coated ELISA plate |
96 holes |
+2 - +8℃ |
Removable and ready to use |
|
Sample Diluent (20×concentrate) |
15ml |
+2 - +8℃ |
High concentrated |
|
Washing solution (20×concentrated) |
30ml |
+2 - +8℃ |
High concentrated |
|
Chloromycin antibody solution |
7ml |
+2 - +8℃ |
Ready to use |
|
Chloromycin standard solution |
0.8ml/tube×5 |
+2 - +8℃ |
Ready to use |
|
High concentration chlortetracycline standard solution |
1ml |
+2 - +8℃ |
High concentrated |
|
Horseradish enzyme-labeled antibody working solution |
7ml |
+2 - +8℃ |
Working concentration, ready to use |
|
TMB substrate solution |
12ml |
+2 - +8℃ |
Single component, ready to use |
|
Stop solution |
12ml |
+2 - +8℃ |
Ready to use |
|
Sealing film |
3 |
|
|
|
Package Insert |
1 |
|
|
[Storage conditions and validity period]
The kit is stored at 2-8°C and is valid for 12 months.
