[Product Usage]
This kit is used to determine the content of total aflatoxin in cereals, flour and other related samples.
[Packaging Specifications]
48T/box, 96T/box
[Principle of experiment]
This kit adopts the indirect competitive ELISA method. Total aflatoxin antigen is pre-coated on the microwell strip of the ELISA plate, and the total aflatoxin remaining in the sample and the antigen pre-coated on the microwell strip compete with the total aflatoxin After adding the enzyme-labeled secondary antibody, the color was developed by the TMB substrate. The absorbance value of the sample was negatively correlated with its total aflatoxin. Compared with the standard curve and multiplied by its corresponding dilution factor, the total amount of the sample in the sample was obtained. Aflatoxin content.
[Main components]
Table 1. Main components of total aflatoxin quantitative detection kit (enzyme-linked immunosorbent assay)
|
Components |
Specification |
Storage condition |
Type |
|
Antigen-coated ELISA plate |
96 holes |
+2 - +8℃ |
Removable and ready to use |
|
Sample Diluent (5×concentrate) |
30ml |
+2 - +8℃ |
High concentrated |
|
Washing solution (20×concentrated) |
30ml |
+2 - +8℃ |
High concentrated |
|
Total aflatoxin antibody solution |
7ml |
+2 - +8℃ |
Ready to use |
|
Total aflatoxin standard solution |
0.8ml/tube×6 |
+2 - +8℃ |
Ready to use |
|
High concentration total aflatoxin standard solution |
1ml |
+2 - +8℃ |
High concentrated |
|
Horseradish enzyme-labeled antibody working solution |
7ml |
+2 - +8℃ |
Working concentration, ready to use |
|
TMB substrate solution |
12ml |
+2 - +8℃ |
Single component, ready to use |
|
Stop solution |
12ml |
+2 - +8℃ |
Ready to use |
|
Sealing film |
3 |
|
|
|
Package Insert |
1 |
|
|
[Storage conditions and validity period]
The kit is stored at 2-8°C, and the validity period is 12 months.
